Cell Culture Insert

Cell culture insert is used in well plates to recreate an original vivo cellular environment for cells. It’s commonly used in the study of cellular functions such as transportation, absorption and secretion.

Cell culture inserts for sale: PC/PET membrane. 6 well, 12 well, 24 well plate. 24 mm, 12 mm, 6.5 mm. 0.4um, 3.0 um, 5.0 um, 8.0um.

MOQ: 1CARTON.

Feature of Cell Culture Insert

High transparency: ensures the clarity of cells under microscope observation.
Surface treatment: specially treated surface can promote cell attachment and growth.
Standard size: suitable for common microscopes and culture equipment, easy to operate.
Multiple specifications: provide different numbers of holes to meet different experimental needs(eg.cell culture inserts 6 well, 12well, cell culture inserts 24 well plate).
Reusable: some models can be disinfected and used multiple times, with a long service life.

Permeable cell culture inserts with PC and PET membrane is available in multiple pore sizes and wells of plate. It is often used with cell culture plate and petri dish.

PET membrane has good light transmittance for observation under microscope. PC membrane has better cell adhesion and higher density of wells, more transmembrane substance can be exchanged.

TC treatment promotes cell attachment; center suspension design prevents damage to cells when they move; fixation and staining can be performed during the experiment;

Precautions for using cell culture plate inserts

Please ensure that the surface of the cell chamber is free of contamination when using.

Avoid the cell chamber from contacting with high temperature or strong acid and alkali substances to avoid damage to the product.

Cell Culture Inserts for Sale

Cat No.	Membrane	Dimensions	Packaging
XBXS-6-PC-24MM-0.4μm	PC	6 well, 24mm, 0.4μm	6 pcs in a plate, 24 pcs/ctn
XBXS-6-PC-24MM-5μm	PC	6 well, 24mm, 5μm	6 pcs in a plate, 24 pcs/ctn
XBXS-6-PET-24MM-0.4μm	PET	6 well, 24mm, 0.4um	6 pcs in a plate, 24 pcs/ctn
XBXS-6-PET-24MM-8μm	PET	6 well, 24mm, 8μm	6 pcs in a plate, 24 pcs/ctn
XBXS-12-PC-12MM-0.4μm	PC	12 well, 12mm, 0.4μm	12 pcs in a plate, 48 pcs/ctn
XBXS-12-PC-12MM-5μm	PC	12 well, 12mm, 5μm	12 pcs in a plate, 48 pcs/ctn
XBXS-12-PET-12MM-0.4μm	PET	12 well, 12mm, 0.4μm	12 pcs in a plate, 48 pcs/ctn
XBXS-12-PET-12MM-3μm	PET	12 well, 12mm, 3μm	12 pcs in a plate, 48 pcs/ctn
XBXS-12-PET-12MM-8μm	PET	12 well, 12mm, 8μm	12 pcs in a plate, 48 pcs/ctn
XBXS-24-PC-6.5MM-0.4μm	PC	24 well, 6.5mm, 0.4μm	12 pcs in a plate, 48 pcs/ctn
XBXS-24-PC-6.5MM-5μm	PC	24 well, 6.5mm, 5μm	12 pcs in a plate, 48 pcs/ctn
XBXS-24-PC-6.5MM-8μm	PC	24 well, 6.5mm, 8μm	12 pcs in a plate, 48 pcs/ctn
XBXS-24-PET-6.5MM-0.4μm	PET	24 well, 6.5mm, 0.4μm	12 pcs in a plate, 48 pcs/ctn
XBXS-24-PET-6.5MM-3μm	PET	24 well, 6.5mm, 3μm	12 pcs in a plate, 48 pcs/ctn
XBXS-24-PET-6.5MM-8μm	PET	24 well, 6.5mm, 8μm	12 pcs in a plate, 48 pcs/ctn
XBXS-6-PC-24MM-5μm-1	PC	6 well, 24mm, 5μm (individual pack)	48 pcs/box, 10 boxes/ctn
XBXS-6-PET-24MM-8μm-1	PET	6 well, 24mm,8μm (individual pack)	48 pcs/box, 10 boxes/ctn
XBXS-12-PET-12MM-8μm-1	PET	12 well, 12mm, 8μm (individual pack)	48 pcs/box, 10 boxes/ctn
XBXS-12-PC-12MM-5μm-1	PC	12 well, 12mm, 5μm (individual pack)	48 pcs/box, 10 boxes/ctn
XBXS-24-PC-6.5MM-5μm-1	PC	24 well, 6.5mm, 5μm (individual pack)	48 pcs/box, 10 boxes/ctn
XBXS-24-PET-6.5MM-8μm-1	PET	24 well, 6.5mm, 8μm (individual pack)	48 pcs/box, 10 boxes/ctn

FAQ of Permeable Cell Culture Inserts

How to choose the right pore size of membrane?

Co-culture experiments usually use 0.4, 3 μm, cell A will be planted in the upper chamber, cell B will be planted in the lower chamber, you can study cell B secretion or metabolism produced by the substances on the cell A effect.
For the caco-2 cell transport model, 0.4 μm membrane was commonly used, and Caco-2 cells were inoculated in Transwell at a density of about 1×104-1×105 cells/mL, 0.5 mL per well, and cultured for about 21 d. The liquid was changed every other day for the first week, and then every day after one week. The transmembrane resistance and alkaline phosphatase activity of the model were monitored to determine whether the model met the requirements of the transporter assay.

Cell migration assay often use 8.0, 12.0μm membrane, the upper chamber seeded cells, the lower chamber to add FBS or some specific chemokines, the cells will be high in nutrients to run to the lower chamber, counting the amount of cells into the lower chamber can reflect the migration ability of the cells.
Cell invasion assay use 8.0, 12.0μm membrane, the principle is similar to the cell migration assay.

What is the difference between PC and PET membranes in application of cell culture plate inserts?

If the experiment requires drug screening or observation of cell morphology, cell culture insert of PC membrane is more suitable because their better light transmission ensures that the experimenter can clearly observe the growth of cells. If the experiment requires dynamic observation of cells under a microscope, cell culture transwel insert of PET film is better, as it is more flexible and can be adjusted to the position and angle of the sample as needed.

How to clean and disinfect the cell chamber?

The permeable cell culture inserts should be cleaned thoroughly after use. It can be disinfected with 75% alcohol or other suitable disinfectants. Avoid using strong acid or alkaline detergents when cleaning to avoid damaging the surface coating of the cell chamber. It is recommended to use non-woven cloth or soft brush to reduce scratches.