Inoculating Loop/Needle

Inoculating loop is a laboratory microbiology tool, widely used in colony streaking, inoculation, transfer of microbial samples and other operations. According to the material, it can be divided into disposable plastic inoculating loop and reusable metal inoculation loop to meet different experimental needs.

Inoculating loops for sale: 1 ul, 10 ul in bulk or individual pack.

MOQ: 1 CARTON.

Feature of  Inoculation Loops

  • Made of pure medical grade HIPS material, good toughness.
  • Smooth surface, no burrs, hydrophilic treatment to reduce cell damage.
  • The other end can be used as an inoculation needle; the inoculation needle tip is rounded to effectively avoid damage to the colony structure during use.
  • 1 ul inoculating loop/10 ul inoculating loop optional, accurate capacity.
  • Produced in a 100,000-level cleanroom, no DNA/RNA enzymes, no pyrogens, no endotoxins.
  • Independent paper-plastic sterile packaging and batch non-sterile packaging are available.

When using this pruduct, it is usually necessary to use some other laboratory tools to ensure the smooth operation. For example, sometimes needs to extract samples from a reagent bottle and transfer them to a petri dish. It can be also used to remove microorganisms from a petri dish and smear them onto a coverslip for observation.

Inoculating Needle vs Inoculating Loop

Inoculating loop and Inoculating needle are two common laboratory tools that are mainly used for inoculation, colony transfer, and sample handling in microbiology. They have similar functions but have some differences in design and application.

Sterile Inoculation Loop

  • Shape and Design: It is usually a thin metal or plastic rod with a ring-shaped portion at the top, which is used to collect colonies or liquid samples. Its ring-shaped design makes it easier to transfer and streak samples.
  • Main Function: Used for inoculation and streaking, especially suitable for colony separation on microbial culture media. The ring design can effectively obtain a certain amount of sample for precise transfer.
  • Common Materials: There are disposable plastic inoculating loops and reusable metal inoculating loops (such as stainless steel or platinum).
  • Applicable Scenarios: Widely used for bacterial culture, fungal inoculation, etc., suitable for relatively “dry” cultures, such as solid culture media.

Inoculating Needle

  • Shape and Design: An inoculating needle is usually a thin, long metal rod with a sharp needle-like portion at the top. It does not have a circular design like a loop, but is in a straight line to help with manipulation.
  • Main Function: Mainly used for streaking and inoculating culture media, and for sampling in liquid or slant culture media. Due to its sharp design, the inoculating needle is often used to remove small amounts of samples and perform more delicate operations.
  • Common Materials: Usually made of metal (such as stainless steel).
  • Applicable Scenarios: Particularly suitable for inoculation operations in slant culture media, because its tip design can accurately contact the sample.

Application areas

  • Microbial inoculation/isolation (bacteria, yeast, fungi).
  • Food, medicine, drinking water microbial testing.
  • Experimental teaching and microbiology training.
  • Clinical bacterial culture and drug sensitivity testing.

Sterile Inoculating Loops for Sale

Cat No. Dimensions Package Weight(KG) Size(CM)
JZH-1P 1ul, bulk pack 10 pcs/bag, 1000 bags/ctn 16.5 43.5×31×43
JZH-1D 1ul, individual pack 1 pcs/bag, 5000 pcs/ctn 12 44×32×44
JZH-10P 10ul, bulk pack 10 pcs/bag, 1000 bags/ctn 16.5 43.5×31×43
JZH-10D 10ul, individual pack 1 pcs/bag, 5000 pcs/ctn 12 44×32×44

FAQ of Bacterial Inoculation Loop

What is the purpose of flaming a loop?

The purpose is to sterilize it, ensuring that no unwanted bacteria remain on the loop.

How is air contamination prevented when inoculating loop is used to introduce a bacterial sample to an agar plate? 

  • Operate in safe environment.
  • Proper sterilization.
  • Use disposable inoculating loops.
  • Avoid ventilation.
  • Precise operation.
  • Use sterilzed containers.

What is the difference between 1ul inoculating loops and 10ul inoculating loops?

The inoculating loop 1ul is suitable for bacterial coating or single bacteria isolation, while the 10 ul inoculating loops is more suitable for quantitative inoculation. Please make the decision based on the experimental requirements.

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What precautions are there for using?

  1. Before use, check whether the sterile packaging is damaged.
  2. If it is a metal ring, it should be sterilized by burning it on an alcohol lamp first, and then contact the sample after cooling.
  3. It should be replaced or re-sterilized in time after each use to avoid cross contamination.

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