PCR Tubes
PCR tubes are commonly used consumables during biological experiments as a container for the polymerase chain reaction. The individual tubes have excellent sealing function with the cap, controlling the loss of reaction volume at 5%, and the thin-wall design makes high heat transfer rate.
Individual PCR tubes for sale: pcr tube flat cap(0.2 ml、0.5ml); 0.2 ml convex cap.
MOQ: 1 CARTON.
0.2 ml PCR Tubes
0.5 ml Pcr Tubes
Feature of PCR Reaction Tube
- Made of heat-resistant medical-grade polypropylene (PP) material with high transparency.
- It is biologically inert and does not easily adhere to biomolecules on its surface.
- Ultra-thin walled design and smooth wall good thermal conductivity.
- Maximum RCF: 6000xg
- Sterile PCR tubes can be used in the temperature range of -20℃~121℃, and withstand 121℃/15psi (15 min) autoclaving.
- Made in class 100,000 clean room, DNase, RNase free pcr tubes without PCR inhibitors, tested Pyrogen free.
- EO ethylene oxide sterilization, SAL 10(-6).
- Customized low adsorptions product is optional to prevent liquid residue.
- Compatible with most standard PCR equipment on the market.
For PCR experiment consumables, we also have PCR strip tube, PCR plate and compatible sealing film.
Tubes PCR for Sale
| Cat No. | Specification | Package | Weight(KG) | Size(CM) |
| PCR1-0.2P | 0.2ml, Flat Cap | 1000pcs/bag, 10bags/ctn | 2.5 | 39×33×19 |
| PCR1-0.2T | 0.2ml, Domed Cap | 1000pcs/bag, 10bags/ctn | 2.5 | 39×33×19 |
| PCR1-0.5 | 0.5ml, Flat Cap | 1000pcs/bag, 10bags/ctn | 5.6 | 29×39×48 |
FAQ of PCR Reaction Tubes
What are pcr tubes used for?
- PCR amplification: commonly used in DNA and RNA amplification experiments.
- Gene expression analysis: suitable for quantitative PCR (qPCR) and RT-PCR experiments.
- DNA template storage: PCR single tubes are also suitable for the storage of genomic DNA and cDNA to ensure the integrity of the sample.
What should I do when the pcr tubes with caps was pushed open due to the pressure difference after the experiment?
Adjust the height of the heat cap before the experiment, pressing the tube is appropriate. If the height is too high, the cap will be pushed out by the airflow inside the tube during the experiment, causing the liquid inside the tube to evaporate and affect the experimental results. The pcr tube strips with attached caps can be opened after the PCR instrument has cooled down to room temperature, so that the temperature difference will be acceptable. It is recommended to set the end temperature to 4 ℃ at the last step.
What are the causes affecting the PCR experiments results?
Primers: Primers are the key to the specificity of the PCR reaction, and the specificity of the PCR product depends on the degree of complementarity of the primer to the template DNA;
Enzyme and its concentration;
dNTP quality and concentration;
template (target gene) nucleic acid;
Mg2+ concentration;
temperature and time settings;
the number of cycles;
equipment, consumables, etc.
What is the maximum volume of pcr tubes?
1. 0.1 mL (100 µL): Ultra-thin-walled tubes designed to improve thermal transfer in real-time PCR.
2. 0.2 mL (200 µL): The standard size for most PCR applications, compatible with many thermal cyclers.
3. 0.5 mL (500 µL): Less common, but still used in some PCR setups, though these larger volumes are generally slower to heat and cool.
The standard type for most applications is the 0.2mL pcr tubes due to its compatibility with a wide range of PCR machines. However, the actual reaction volume is often much lower (10–50 µL), allowing room for thermal expansion and minimizing evaporation.
How to prevent polypropylene pcr tubes from being contaminated during experiments?
To prevent contamination, it is recommended to use sterile pipettes when operating and ensure the cleanliness of the experimental environment. Seal the tubes promptly after use to avoid long-term exposure to the air. You can use a special PCR tube rack to store the tubes to avoid touching unnecessary surfaces.
