Petri Dish

Petri dish is a laboratory dish designed specifically for microbial research. It is mainly used to culture, isolate and observe bacteria, fungi and other microorganisms in a sterile environment.

Petri dish for sale: 35 mm, 60 mm, 70 mm, 75 mm, 90 mm, 100 mm, 120 mm, 150 mm of round dish.
100 mm and 130 mm of square petri dish.Petri dishes with ears or compartments are also available.

MOQ: 1 CARTON.

Petri Dishes for Sale

Cat No. Detail Packaging Weight Size
PYM-35 35mm 10 pcs/bag, 200 bags/ctn 10.8 41×45×32
PYM-60 60mm 10 pcs/bag, 100 bags/ctn 13.6 65×35×36
PYM-70 70mm 10 pcs/bag, 80 bags/ctn 11.9 60×35×38
PYM-75 75mm with nesting lugs 10 pcs/bag, 36 bags/ctn 8.4 52×32×44
PYM-9012 90x15mm12g 10 pcs/bag, 50 bags/ctn 8.3 49×49×36
PYM-9015 90x15mm15g 10 pcs/bag, 50 bags/ctn 8.6 49×49×36
PYM-9016 90x16mm16g 10 pcs/bag, 50 bags/ctn 8.7 46.5×46.5×34.5
PYM-9020 90x20mm 10 pcs/bag, 50 bags/ctn 8.7 49×49×42
PYM-902 90mm, 2-part grid 10 pcs/bag, 50 bags/ctn 8.6 49×49×36
PYM-903 90mm, 3-part grid 10 pcs/bag, 50 bags/ctn 8.7 49×49×36
PYM-904 90mm, 4-part grid 10 pcs/bag, 50 bags/ctn 8.8 49×49×36
PYM-100 100mm 10 pcs/bag, 32 bags/ctn 7.1 43×44×38
PYM-120 120mm 10 pcs/bag, 24 bags/ctn 7.5 52×38×47
PYM-150 150mm 10 pcs/bag, 20 bags/ctn 8.6 77×34×35
PYM-15020 150mm*20mm 10 pcs/bag, 20 bags/ctn 8.6 77×34×45
PYM-100F 100mm, square 10 pcs/bag, 40 bags/ctn 7.1 57×46×39
PYM-130F 130mm, square 10 pcs/bag, 30 bags/ctn 8.1 57×46×52

Feature of Petri Dish for Bacteria

 
  • Made of pure imported medical grade polystyrene (PS) material, high transparency.
  • Smooth surface without distortion, uniform thickness, no optical distortion.
  • Stacking design, saves space and is easy to store.
  • TC treated, excellent wall adhesion.
  • Produced in a 100,000-level clean workshop, no DNA/RNA enzymes, no pyrogens.
  • EO ethylene oxide/irradiation sterilization,SAL 10(-6).
  • Various sizes/square petri dish/with ears or compartments are also available.
  • The available bacteria petri plate sizes include: 35 mm petri dish~150mm round dish, 75mm with nesting lug and 90mm with compartments. 100mm petri dish and 130 square petri dish. 

FAQ of Petri Dish

Can plastic petri dishes be autoclaved?

Standard polystyrene petri dishes are generally not suitable for autoclaving because of their limited heat resistance. Follow the manufacturer’s instructions and use only products specifically validated for the intended sterilization method.

Petri dishes are commonly used for microbiological culture and general sample handling, while cell culture dishes are specifically designed for cell culture applications and may feature tissue culture-treated surfaces to promote cell attachment.

Store petri dishes in their original packaging in a clean, dry environment, away from excessive heat, direct sunlight, and contamination. Sterile petri dishes should remain sealed until use.

During cultivation, more water vapor will be produced in the petry dish. The water vapor will condense on the dish cover and produce water droplets. If the dish is placed upright, the dripping water will disperse the bacterial colonies. In this case, a large bacterial colony may disperse and become Many small colonies cause a lot of trouble in culturing and counting bacteria. If it is turned upside down, with the medium on top and the dish cover on the bottom, water droplets will not drip onto the colonies.

1. Preparation of Materials

  • Sterilized Petri dishes .
  • Nutrient agar or LB agar (pre-mixed or prepare by dissolving agar powder in distilled water).
  • Autoclave (for sterilizing media at 121°C for 15–20 minutes).
  • Inoculation loop, Bunsen burner, pipettes, and a incubator (set to the target temperature, e.g., 37°C for E. coli).

2. Prepare Agar Plates

  • Sterilize the medium: Autoclave the agar mixture, then cool to ~50°C (warm to touch but not hot).
  • Pour the agar: In a sterile environment (e.g., laminar flow hood), pour 15–20 ml of melted agar into each petri dish. Let solidify completely (20–30 minutes).

3. Inoculate the Bacteria

  • Sterilize tools: Flame the inoculation loop until red-hot, then cool briefly.
  • Transfer bacteria: For streak plating: Dip the loop in a bacterial sample, then streak the agar surface in a zigzag pattern to isolate colonies. For spread plating: Add 0.1–0.2 ml of diluted bacterial suspension to the agar, then spread evenly with a sterile spreader.

4. Incubation

  • Invert the plates (to prevent condensation from dripping onto colonies).
  • Place in an incubator at the optimal temperature (e.g., 37°C for 24–48 hours for most bacteria).

5. Observe Results

  • Check for bacterial colony formation (size, shape, color, and distribution).

One-stop laboratory consumables supplier

Get free samples