Cell Strainer

Cell strainer is manufactured of a plastic or nylon that pass fluids through and retain large particle in the mesh for multiple applications: filter and separate cells, remove clumps or debris.

Cell strainer for sale: 150 mesh 100μm, 220 mesh 70μm, 330 mesh 40μm.

MOQ: 1 CARTON.

Cell Strainer for Sale

Cat No.	Dimensions	Package
XBSW-150-100μm	100μm, 150 mesh, green	1 pcs/bag，100 pcs/ctn
XBSW-220-70μm	70μm, 220 mesh, orange	1 pcs/bag，100 pcs/ctn
XBSW-330-40μm	40μm, 330 mesh, purple	1 pcs/bag，100 pcs/ctn

Feature of Cell Strainer

Made with a durable polypropylene (PP) frame and a nylon mesh for reliable performance and long-lasting durability.
Designed to remove cell clumps and debris from cell suspensions, helping obtain a more uniform cell suspension.
Facilitates cell suspension filtration and helps simplify laboratory sample preparation workflows.
Suitable for preparing single-cell suspensions for cell culture, flow cytometry, and other cell-based research applications.
Available in different mesh sizes to accommodate various cell types and sample preparation requirements. 40um cell strainer, 70 um cell strainer,100 μm cell strainer are available.
Designed for convenient handling during routine cell filtration and sample preparation procedures.

FAQ of Cell Filter

Do cell strainers affect cell viability?

Cell viability may be affected by sample handling, filtration pressure, mesh size, and the characteristics of the cells. Use an appropriate mesh size and gentle handling to help minimize cell damage.

Can cell strainers be used with centrifuge tubes?

Available in formats designed to fit 50ml centrifuge tubes, depending on the product model.

What are the precautions when filter cells?

For the screening of adherent cells, the strainer can be turned over and then filtered with a filter containing cell culture medium, and the filtered medium can be collected and used for cell culture.
During the operation, it is better to carry out the experiment under aseptic conditions in order to ensure the sterility of the experiment and to avoid the contamination of air and external substances.

Can cell strainers be used for flow cytometry?

Yes. Cell strainers are commonly used to prepare single-cell suspensions before flow cytometry to help reduce clumps that may interfere with sample analysis.

How do I choose the right cell strainer mesh size?

The appropriate cell strainer mesh size depends on the target cell size, sample characteristics, and downstream application.

Cell strainer 40 um: Suitable for finer filtration of cell suspensions, helping remove small clumps and debris while retaining cells larger than the mesh openings. Commonly used for lymphocytes, blood cells, and certain stem cell preparations.
70um cell strainer: A common choice for routine single-cell suspension preparation, including samples containing fibroblasts and certain tumor cells.
100um cell strainer: Suitable for samples containing larger cell aggregates or tissue fragments, such as digested tumor tissues and primary tissue preparations, where a larger mesh opening is preferred.

How to gain individual mouse spleen cells of good avtivity?

To obtain fresh mouse spleens which are oval shaped and can be separated directly by pulling with forceps, firstly place the mouse spleen in a pre-cooled petri dish or medium-sized centrifuge tube with appropriate amount of HBSS (Hanks’ Balanced Salt Solution) buffer and use surgical scissors or ophthalmic scissors to carefully cut up the spleen.
Place a cell sieve over a 50 mL centrifuge tube and, using a disposable pipette, transfer the clipped spleen to the cell sieve by gently mashing or crushing it to pass through the sieve. If necessary, add 5-10 mL of PBS to rinse. Repeat the above steps, which can be verified using a 1 mL syringe. Single-cell suspension preparation is considered complete if the cell suspension that passes through the sieve can be blown repeatedly using a syringe (with a needle) without obstruction.
Centrifuge the cell suspension at 400-600 g or 1500 rpm for 10 min at 4°C. Discard the supernatant and resuspend the cells in 2-5 mL of pre-cooled 1x PBS. Put the resuspension solution on ice, if necessary, the cells can be stained with Taipan blue to determine the viability, or single cell counting of the cells, adjusted to the appropriate concentration of cells, according to the purpose of the assay experiment were stained, on the machine for testing.